Association of Mitochondrial DNA 4977bp Deletion and Total Antioxidant Capacity with Insulin Resistance in Healthy Iraqi Subjects
This study is to determine if there is an association between the mitochondrial DNA (mtDNA) 4977 deletion and the development of insulin resistance in Iraqi healthy subjects. two hundred apparently healthy individuals (women and men), at age < 45 at the Research Unit, College of Medicine, Al-Nahrain University. Individuals were divided into insulin-sensitive (group A) and insulin resistance (group B). The study comprised euglycemic non-diabetic individuals with an HbA1C of less than 6.5%. Fasting insulin was done using a competitive enzyme-linked immunosorbent assay (ELISA) technique. Colorimetric methods were used to estimate lipid profiles. DNA extraction was done using a Genomic DNA G-spin kit supplied by Intron Biotechnology, Cat. No. 17045. polymerase chain reaction (PCR) amplification was done for all subjects using specific primer pairs. Regarding the demographic factors with Insulin Resistance, significant differences were found in the means of age and BMI between groups (A) and (B). High levels were found in the means of HbA1c, Fasting insulin, and (Homeostatic Model Assessment for Insulin Resistance) HOMOA-IR. In the B group compared to group A, by using ROC to evaluate the diagnostic value of TAOC, the curve (AUC) was 0.755, 95%CI= 0.623-0.866, p=0.004. The test's sensitivity and specificity were 71% and 62%, respectively. The best cut-off value of TAOC was 0.41 U/ml. Finally, no mtDNA 4977 deletion was found in Iraqi subjects of the two groups. All Iraqi subjects in this study didn’t have mtDNA 4977 deletion, so the development of IR can be attributed to many other causes discussed below.