Hub genes associated with peptidyl lysine-acetylation as a new therapeutic insight for irritable bowel syn-drome and Parkinson’s disease: data analytic ap-proaches
Dysbiosis in the gut microbiome may cause neurological and gastrointestinal conditions, impairing cognitive and immune functions. Increased evidence suggested that irritable bowel syndrome (IBS) patients are at higher risks in developing Parkinson’s Disease (PD). This study aimed to predict gene signatures and potential molecular networks linking IBS with PD. A total of five microarray datasets were obtained online at gene database Gene Expression Omnibus (GEO): GSE13367, GSE36701, GSE49126, GSE99039, and GSE28894. The differentially expressed genes (DEGs) were chosen for both PD and IBS based on log2FC >1.0 and p <0.05. Gene ontology (GO) enrichment analysis was studied through DAVID to perform additional analysis on the shared DEGs. The predictive protein-protein interaction (PPI) network was subsequently built using GeneMANIA to illustrate the interactions and support functional prediction for IBS and PD. In total, 12748 DEGs and 165 DEGs were identified from IBS and PD samples, respectively. The GO enrichment analysis of 87 commonly shared DEGs exhibited multiple shared biological processes, cellular comportments, and molecular function between the selected genes. The top 10 biological processes' DEGs were chosen to form the PPI network and possibly identify the co-expression and pathways associated with IBS and PD. This study found that CREBBP, LEF1, RUVBL1, KAT2B and RPS6KA5 were involved in the pathogenesis of both PD and IBS, primarily through peptidyl-lysine acetylation.