Preparation and characterization of a new azo ligand with platinum (IV) complex and evaluation of their biological activity as anti-liver cancer substances.
The research includes the preparation of a new homocyclic azo ligand, which is 6,6 – ((methylene bis (4,1-phenylene)) bis (diazene-2,1-dil)) bis (4-chloro-2-nitrophenol) By dissolving (4,4'-Methylenedianiline) with Sodium nitrite and forming the diazonium salt, which in turn enters the coupling reaction with (4-chloro-2-nitrophenol) Then the solid metal complex of the azo ligand with platinum (IV) ion was prepared. The prepared ligand and complex were characterized by infrared and ultraviolet spectroscopy. The biological effect of the prepared complex and ligand and the possibility of using them in the medical aspect as a drug were studied. The study included two types of pathogenic bacteria isolated and laboratory-diagnosed using chemical and microscopic tests, Gram-positive Streptococci and Gram-negative Escherichia, and two types of fungi Aspergillus niger and Penicillium sp. The toxicity levels of some of the compounds under study were also studied, as the effect of the azo ligand and the platinum complex on the growth of liver cancer cell lines HepG2 and healthy cells WRL-68 was studied for the purpose of knowing their toxic effectiveness on the cells of the human body and the possibility of using them as a treatment for liver cancer.