Selection of bacterial strains with biofilm-forming potential for applications in oil spill remediation in aquatic environments
This study focuses on the selection of bacterial strains with strong biofilm-forming abilities and their application potential in oil spill remediation within aquatic environments. Nine bacterial strains, including Pseudomonas aeruginosa, Alcanivorax borkumensis, and Rhodococcus erythropolis, were evaluated for biofilm formation and hydrocarbon degradation efficiency. Experimental results revealed that these strains exhibited significant biofilm formation and efficient degradation of petroleum hydrocarbons, particularly at an optimal incubation temperature of 40°C over 72 hours. Antagonistic tests further identified compatible strain combinations, selecting four strains—T1, T2, T6, and T8—for further application. This combination of strains demonstrates high potential for creating a biochar formulation capable of effectively degrading diesel oil in contaminated water, making it a promising solution for oil spill remediation under field conditions, especially in environments exposed to high temperatures.