Synthesis and spectral identification of the Au(III) complex with a new azo dye and assessment of its effect on the expression of PARP, a DNA repair enzyme, in breast cancer
A new ligand, 2-[2 '-(6-methoxybenzothiazolyl)azo]-4-methyl phenol (6-MBTAMP), a derivative of 2-amino-6-methoxy benzothiazole, has been used to synthesize a new metal complex of Au(III). The metal complex was characterized by spectroscopic techniques: UV-Vis, mass spectrometry, FT-IR, 1H NMR, and FE-SEM. As well as elemental analyses (C.H.N.S), crystal structure (XRD). Results shown that the azo dye ligand behaves a tridentate and coordinates to the metal ion via the nitrogen atom of the azomethine group of the heterocyclic benzothiazole ring, the nitrogen atom of the azo group which is the farthest of the benzothiazole molecule and the oxygen atom of the hydroxyl. Molecular docking was used to determine the effectiveness of these compounds are against cancer. (6-MBTAMP) and the Au(III) complex were examined for their cytotoxicity in breast cancer (MCF-7(, the antioxidant and anticancer activities were performed by assessing of MCF-7 proliferation, and the intracellular contents of catalase and malondialdehyde (MDA) levels) and were compared with those of the non-treated MCF-7 cell line. The expression levels of the DNA repair enzyme, poly(ADP-ribose) polymerase (PARP) were also investigated. Moreover, one hundred twenty participants 60 women with breast cancer and 60 controls were enrolled n this study. The change in oxidant-antioxidant system were also determined by measuring MAD and catalase levels in addition to evaluating the changes in the gene expression levels of PARP. Interestingly, the levels of PARP were reversed in MCF-7 cells treated with the complex.