Synthesis, identification, spectroscopic and anticancer studies of heterocyclic azo ligand derived from 2-Amino benzimidazole with gold metal ions and palladium metal ions
This study included the synthesis of E-2- ((1H-benzo[d]imidazol-2-yl)diazenyl)-5-methyl-4-nitrophenol (BIDAMeNP), Au(III) complex and its Pd(II) complex. Various analytical techniques, including FT-IR, XRD, TGA, DTA, UV-VIS spectroscopy, Mass spectrometry,1H-NMR and 13C-NMR were utilized for compound characterization. Cytotoxicity of both the ligand BIDAMeNP and gold(III). Complex against human MCF7 breast cancer-cells was assessed via MTT assay. The ligand (BIDAMeNP) demonstrated significant inhibitory effects on MCF7 cells, achieving an IC50 value of 188.2 ppm while the gold(III)Complex exhibited lower efficacy with an IC50 value of 89.09 ppm compared to the ligand (BIDAMeNP). ). we benchmarked the results against ascorbic acid for reference Results were benchmarked against ascorbic acid for reference. Furthermore, the synthesized ligand, Au(III)-complex and Pd(II) complex the specimens underwent assessment for their antibacterial and antifungal properties. Their activity against specific strains, including Candida Aspergillus niger, Escherichia coli and Staphylococcus. , the results showed high biological effectiveness of the BIDAMeNP ligand, Au(Ⅲ) complex and its Pd(Ⅱ) complex on the types of bacteria and fungi that cause many common diseases, and these results bode well. Additionally, the antioxidant properties of the compounds were investigated. These findings suggest the potential of the synthesized compounds as anti-cancer agents especially breast cancerand warrant further exploration in the field of breast cancer pharmacology.